Signatories include joint chief executives and general directors of the Welsh National Opera (WNO), Adele Thomas and Sarah Crabtree, the founder of the Vagina Museum, Florence Schechter, and the director of Queer Britain museum, Andrew Given. The letter says that the majority of cultural venues "are unable to magic up new toilet facilities" and "this kind of segregation will have significant social, cultural and economic impact". The Supreme Court judges say trans people are still protected from discrimination under equalities legislation, and that this interpretation of the law does not cause disadvantage to the "potentially vulnerable group".

The court sided with campaign group For Women Scotland, which brought a case against the Scottish government and argued for a "common sense" interpretation of the words man and woman, telling the court that sex is an "immutable biological state". Following the ruling two weeks ago, For Women Scotland co-founder Susan Smith said: "Sex is real and women can now feel safe that services and spaces designated for women are for women and we are enormously grateful to the Supreme Court for this ruling." Harry Potter author JK Rowling posted on social media to say the campaign group have "protected the rights of women and girls across the UK."

Barrister Akua Reindorf described the ruling as, external "a model of clarity and provides a solid foundation for approaching consequential issues", while Helen Joyce, the director of advocacy at the campaign group Sex Matters, said the ruling was "incredibly important for the half of humanity who need single-sex spaces". Several organisations have been updating their guidelines in light of the Supreme Court's ruling. Earlier this week, the Football Association announced transgender women will no longer be able to play in women's football in England from 1 June.